Nanog Independent Reprogramming to iPSCs with Canonical Factors

ABSTRACT: It has been suggested that the transcription factor Nanog is essential for the establishment of pluripotency during the derivation of embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. However, successful reprogramming to pluripotency with a growing list of divergent transcription factors, at ever increasing efficiencies, suggests that there may be many distinct routes to a pluripotent state. Here, we have investigated whether Nanog is necessary for reprogramming murine fibroblasts under highly efficient conditions using the canonical reprogramming factors Oct4, Sox2, Klf4 and cMyc. In agreement with prior results, the efficiency of reprogramming Nanog-/- fibroblasts was significantly lower than that of control fibroblasts. However, in contrast to previous findings, we wer

ORGANISM(S): Mus musculus

SUBMITTER: Brandi Davis-Dusenbery 

PROVIDER: E-GEOD-53212 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress