Differential expression of genes in the WT and hupB KO strain of Mycobacterium tuberculosis H37Rv upon iron limitation

ABSTRACT: HupB is a 28 kDa in Mycobacterium tuberculosis that is co-expressed with the siderophores mycobactin and carboxymycobactin upon iron limitation. High levels of all the three components are seen in low iron (LI; 0.02 M-BM-5g Fe / mL) organisms, with negligible expression in high iron organisms (HI; 8 M-BM-5g Fe / mL). We generated a hupB knock out mutant of M. tuberculosis (H37Rv M-bM-^HM-^F hupB) and studied the differential expression of genes upon iron limitation in the WT H37Rv and the mutant. The RNA transcripts of the WT H37Rv, grown under high and low iron conditions of growth were isolated and subjected to microarray analysis to identify the iron-regulated genes and second, the differential expression of genes in iron-limited H37Rv M-bM-^HM-^F hupB vs iron-limited WT H37Rv was anal

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Manjula Sritharan 

PROVIDER: E-GEOD-53254 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress