Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Molecular signatures of the evolving immune response in mice following a Bordetella pertussis infection.

ABSTRACT: Murine gene expression responses to Bordetella pertussis were determined in lung and spleen, between 0 and 28 days post infection. Data were compared to other parameters such as microarray, flow cytometry, multiplex immunoassays, and lung clearance. A 57 array study with 27 arrays for lung and 30 for spleen. For each tissue we used a common reference made from RNA samples of the same tissue. Please note that the reference samples are made by pooling RNA of all samples used for a tissue (either infected or uninfected). Thus, Lung and spleen each have their own reference.

ORGANISM(S): Mus musculus

SUBMITTER: Jeroen Pennings

PROVIDER: E-GEOD-53294 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Molecular signatures of the evolving immune response in mice following a Bordetella pertussis infection.

Raeven René H M RH Brummelman Jolanda J Pennings Jeroen L A JL Nijst Olaf E M OE Kuipers Betsy B Blok Laura E R LE Helm Kina K van Riet Elly E Jiskoot Wim W van Els Cecile A C M CA Han Wanda G H WG Kersten Gideon F A GF Metz Bernard B

PloS one 20140819 8

Worldwide resurgence of pertussis necessitates the need for improvement of pertussis vaccines and vaccination strategies. Since natural infections induce a longer-lasting immunity than vaccinations, detailed knowledge of the immune responses following natural infection can provide important clues for such improvement. The purpose was to elucidate the kinetics of the protective immune response evolving after experimental Bordetella pertussis (B. pertussis) infection in mice. Data were collected f ...[more]

PMID: 25137043

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Project description:Cellular stress responses are frequently presumed to be more sensitive than traditional ecotoxicological life cycle endpoints such as survival and growth. Yet, the focus to reduce test duration and to generate more sensitive endpoints has caused transcriptomics studies to be performed at low doses during short exposures, separately and independently from traditional ecotoxicity tests, making comparisons with life cycle endpoints indirect. Therefore we aimed to directly compare the effects on growth, survival and gene expression of the non-biting midge Chironomus riparius. To this purpose, we analyzed simultaneously life cycle and transcriptomics responses of chironomid larvae exposed to four model toxicants. We observed that already at the lowest test concentrations many transcripts were significantly differentially expressed, while the life cycle endpoints of C. riparius were hardly affected. Analysis of the differentially expressed transcripts showed that at the lowest test concentrations substantial and biologically relevant cellular stress was induced and that many transcripts responded already maximally at these lowest test concentrations. The direct comparison between molecular en life cycle responses after fourteen days of exposure revealed that gene expression is more sensitive to toxicant exposure than life cycle endpoints, underlining the potential of transcriptomics for ecotoxicity testing and environmental risk assessment. Cellular stress responses are frequently presumed to be more sensitive than traditional ecotoxicological life cycle endpoints such as survival and growth. Yet, the focus to reduce test duration and to generate more sensitive endpoints has caused transcriptomics studies to be performed at low doses during short exposures, separately and independently from traditional ecotoxicity tests, making comparisons with life cycle endpoints indirect. Therefore we aimed to directly compare the effects on growth, survival and gene expression of the non-biting midge Chironomus riparius. To this purpose, we analyzed simultaneously life cycle and transcriptomics responses of chironomid larvae exposed to four model toxicants. We observed that already at the lowest test concentrations many transcripts were significantly differentially expressed, while the life cycle endpoints of C. riparius were hardly affected. Analysis of the differentially expressed transcripts showed that at the lowest test concentrations substantial and biologically relevant cellular stress was induced and that many transcripts responded already maximally at these lowest test concentrations. The direct comparison between molecular en life cycle responses after fourteen days of exposure revealed that gene expression is more sensitive to toxicant exposure than life cycle endpoints, underlining the potential of transcriptomics for ecotoxicity testing and environmental risk assessment. Four 14-day Chironomus riparius sediment toxicity tests were conducted, one with each toxicant. The surviving larvae were individually flash frozen in liquid nitrogen. For each toxicant we analyzed the gene expression of larvae exposed to low, intermediate and high concentrations. We also included a control and a solvent control. For each treatment we analyzed 10 replicates (individual larvae).

Dataset Information

Molecular signatures of the evolving immune response in mice following a Bordetella pertussis infection.

Publications

Molecular signatures of the evolving immune response in mice following a Bordetella pertussis infection.

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