Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Drosophila melanogaster selected for aggressive behavior vs non aggressive control

ABSTRACT: We generated 4 separate subpopulations of selected flies from 1 starting population of a mixture of Canton-S flies. Two of the populations (AggrI and AggrII) were selected for increased aggression by picking males from a population cage that engaged in the most intense fighting (known as escalation behavior). Thirty males were selected every generation (from a total of 120 males per cage) per line and mated with random virgin females from that same generation. Two control populations (NeutrI and NeutrII) were selected by picking random males from the population cage after 15-30 aggressive males were removed. After 21 generations of selection males from the Aggr lines were dramatically more aggressive than males from the Neutr lines. The Neutr lines did not significantly differ from the starting population although a trend of decreased aggression was visible. Experiment Overall Design: Four populations were analyzed. Two were selected for increased aggression and two were reference groups, selected for a decrease but with no significant effect.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Herman Albert Dierick

PROVIDER: E-GEOD-5335 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Publications

Molecular analysis of flies selected for aggressive behavior.

Dierick Herman A HA Greenspan Ralph J RJ

Nature genetics 20060813 9

Aggressive behavior is pervasive throughout the animal kingdom, and yet very little is known about its molecular underpinnings. To address this problem, we have developed a population-based selection procedure to increase aggression in Drosophila melanogaster. We measured changes in aggressive behavior in the selected subpopulations with a new two-male arena assay. In only ten generations of selection, the aggressive lines became markedly more aggressive than the neutral lines. After 21 generati ...[more]

PMID: 16906161

Similar Datasets

Project description:This study measured the differential expression of genes from two populations of Wyeomyia smithii in the USA that differ in their propensity to bite over a geographic gradient. Population KC is a northern non-biting population from Maine; population WI is a southern low-frequency biting population from Florida. The mosquitoes were maintained at the University of Oregon under standard rearing conditions and run through two generations to minimize maternal and field effects. Selection for biting was conducted using ~14,000 individuals from the WI population. The environment and protocols used for selection were the same as for KC except biters were removed from their cage and placed into a separate “biting” cage with supplemental males from the same generation of the selected line. All hatch from the biting cage were used to generate the subsequent generations. This protocol was followed until the selected line had undergone 5 generation of selection. Through all generations of selection, including those that were not offered a host, hatch were placed on short days (LL:D=10:14) at 21°C to synchronize each generation and to miigate inadvertant direct selection on development time, generation time, or the timing of reproductive allocation. After adults of a given generation had died, their offspring were transferred to long days and reared to adulthood, as above. During experiments, all animals were in good physical condition. Animal heads were dissected then transferred into 1.5-ml microcentrifuge tubes and shock-frozen in liquid nitrogen. Samples were short-term stored at -80°C. For all experimental conditions, four independent replicates (50 heads each) were analyzed. This GEO record is for three comparisons among the three populations: (EXP1) non-biters in the base population (WI) versus biting individuals in the WI line selected for biting (WIOB) over 5 generations of selection (WI vs WIOB); (EXP2) WIOB versus northern obligate non-biters (KC); (3) WI versus KC (n=4 per treatment group for each population).

Project description:Aggression among group housed male mice continues to challenge laboratory animal researchers because mitigation strategies are generally applied at the cage level without a good understanding of how it affects the dominance hierarchy. Aggression is typically displayed by the dominant mouse, targeting lower ranking subordinates, thus strategies may be more successful if applied specifically to the dominant mouse. Unfortunately, dominance rank is often not assessed because of time intensive observations or tests. Several dominance measures have been developed, but none directly compared to home cage behavior in standard housing. This study assessed the convergent validity of three dominance measures (urinary darcin, tube test score, preputial gland to body length ratio) with wound severity and rankings based on home cage behavior, using factor analysis. Discriminant validity with open field measures was assessed to determine if tube test scores are independent from anxiety. Cages were equally split between SJL and albino C57BL/6 strains and group sizes of 3 or 5 (N=24). During the first week, home cage behavior was observed, and the dominance measures were recorded over the second week. After controlling for strain and group size, darcin and preputial ratio had strong loadings on the same factor as home cage ranking and were significant predictors of home cage ranking showing strong convergent validity. Tube test scores were not significantly impacted by open field data, showing discriminant validity. Social network analysis was also done to reveal that despotic power structures were prevalent, aggressors were typically more active and rested away from cage mates, and the amount of social investigation and aggression performed by an individual were highly correlated. Data from this study show that darcin and preputial ratio are representative of home cage aggression and provide further insight on individual behavior patterns in group housed male mice.

Dataset Information

Transcription profiling of Drosophila melanogaster selected for aggressive behavior vs non aggressive control

Publications

Molecular analysis of flies selected for aggressive behavior.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure