High Resolution Copy Number Analysis of Paraffin Embedded Archival Tissue Using SNP BeadArrays

ABSTRACT: High density SNP microarrays provide insight into the genomic events that occur in diseases like cancer by their capability to measure both LOH and genomic copy numbers. Where currently available methods are restricted to the use of fresh frozen tissue, we now describe the design and validation of copy number measurements using the Illumina BeadArray platform and its application to formalin fixed, paraffin embedded (FFPE) tissue. In fresh frozen tissue, using a set of colorectal tumors with numerous chromosomal aberrations, our method measures copy number patterns that are comparable to values from established platforms, like Affymetrix GeneChip and BAC array-CGH. Moreover, paired comparisons of fresh frozen and FFPE tissue showed nearly identical patterns of genomic changes. We conclude t

ORGANISM(S): Homo sapiens

SUBMITTER: Jan Oosting 

PROVIDER: E-GEOD-5347 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress