PRC2 coordinates lineage fidelity and DNA methylation during ESC differentiation (RRBS)

ABSTRACT: Polycomb Repressive Complex 2 (PRC2) catalyzes histone H3 lysine 27 tri-methylation, an epigenetic modification associated with gene repression. H3K27me3 is enriched at the promoters of a large cohort of developmental genes in embryonic stem cells (ESCs). Loss of H3K27me3 leads to a failure of ESCs to properly differentiate, which presents a major roadblock for dissecting the precise roles of PRC2 activity during lineage commitment. While recent studies suggest that loss of H3K27me3 leads to changes in DNA methylation in ESCs, how these two pathways coordinate to regulate gene expression programs during lineage commitment is poorly understood. Here, we analyzed gene expression and DNA methylation levels in several PRC2 mutant ESC lines that maintain varying levels of H3K27me3. We found tha

ORGANISM(S): Mus musculus

SUBMITTER: Laurie Boyer 

PROVIDER: E-GEOD-53507 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress