Genome-wide activity of unliganded Estrogen Receptor alpha in breast cancer cells [ChIP-Seq]

ABSTRACT: Estrogen Receptor ? (ER?) has central role in hormone-dependent breast cancer and its ligand-induced functions have been extensively characterized. However, evidence exists that ER? has functions which are independent of ligands. In the present work, we investigated the binding of ER? to chromatin in absence of ligands, and its function(s) on gene regulation. We demonstrated that in MCF7 breast cancer cells unliganded ER? binds to more than four thousands chromatin sites. Unexpectedly, although almost entirely comprised in the larger group of estrogen-induced binding sites, we found that unliganded-ER? binding is specifically linked to genes with developmental functions, as compared to estrogen-induced binding. Moreover, we found that siRNA-mediated downregulation of ER? in absence of estr

ORGANISM(S): Homo sapiens

SUBMITTER: Giulio Ferrero 

PROVIDER: E-GEOD-53531 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress