SIRT1 affects DNA methylation of polycomb group protein target genes (PCGTs), a hotspot of the epigenetic shift observed in ageing

ABSTRACT: Beneficial effects of SIRT1 on healthspan are likely to be pleiotropic and may include effects on DNA methylation. We demonstrated recently that manipulating SIRT1 in human cells affected DNA methylation of a panel of test genes, and that genes with expression modified by dietary restriction corresponded with genes that underwent changes in DNA methylation during ageing. Here we tested the hypothesis that genes particularly susceptible to SIRT1-induced effects on DNA methylation across the genome map to genes for which DNA methylation changes during ageing. We increased or reduced SIRT1 expression in human intestinal (Caco-2) and vascular endothelial (HuVEC) cells by transient transfection with an expression construct or with siRNA, respectively. Effects on DNA methylation were measured by

ORGANISM(S): Homo sapiens

SUBMITTER: Simon Cockell 

PROVIDER: E-GEOD-53569 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress