Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPaseold-35)

ABSTRACT: Human Polynucleotide Phosphorylase (hPNPaseold-35) is an evolutionarily conserved 3’?5’ exoribonuclease implicated in the regulation of numerous physiological processes like maintenance of mitochondrial homeostasis, mtRNA import and aging-associated inflammation. From an RNase perspective, little is known about the RNA or miRNA species it targets for degradation or whose expression it regulates; except for c-myc and miR-221. To further elucidate the functional implications of hPNPaseold-35 in cellular physiology, we knocked-down and overexpressed hPNPaseold-35 in melanoma and HeLa cells, respectively, and performed gene expression analyses to identify differentially expressed transcripts. Biological triplicates were run on microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Catherine Dumur

PROVIDER: E-GEOD-53604 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Publications

Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPase(old-35)).

Sokhi Upneet K UK Bacolod Manny D MD Emdad Luni L Das Swadesh K SK Dumur Catherine I CI Miles Michael F MF Sarkar Devanand D Fisher Paul B PB

Journal of cellular physiology 20141201 12

As a strategy to identify gene expression changes affected by human polynucleotide phosphorylase (hPNPase(old-35)), we performed gene expression analysis of HeLa cells in which hPNPase(old-35) was overexpressed. The observed changes were then compared to those of HO-1 melanoma cells in which hPNPase(old-35) was stably knocked down. Through this analysis, 90 transcripts, which positively or negatively correlated with hPNPase(old-35) expression, were identified. The majority of these genes were as ...[more]

PMID: 24729470

Dataset Information

Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPaseold-35)

Publications

Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPase(old-35)).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Similar Datasets

Gene Expression Signature of Human Polynucleotide Phosphorylase (hPNPaseold-35) in Melanoma
2013-07-30 | E-GEOD-46884 | biostudies-arrayexpress

Gene Expression Signature of Human Polynucleotide Phosphorylase (hPNPaseold-35) in Melanoma
2013-07-30 | GSE46884 | GEO

Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPaseold-35)
2014-06-01 | GSE53604 | GEO

Global analysis of mRNA decay intermediates in Bacillus subtilis wild-type and polynucleotide phosphorylase-deletion strains
| PRJNA491499 | ENA

Polynucleotide phosphorylase promotes the stability and function of Hfq-binding sRNAs by degrading target mRNA-derived fragments
| PRJNA515929 | ENA

Polynucleotide phosphorylase promotes the stability and function of Hfq-binding sRNAs by degrading target mRNA-derived fragments
2019-07-08 | GSE125368 | GEO