Decoding the black box of cellulase formation in Hypocrea jecorina by RNA-Seq analysis

ABSTRACT: Hypocrea jecorina (anamorph Trichoderma reesei) is one of the most well studied fungi used in biotechnology industry. This fungus is today a paradigm for the comercial scale production of different plant cell wall degrading enzymes, mainly cellulases and hemicellulases. The objective of this study was to analyze the transcriptional profiling of T. reesei grown in presence of cellulose, sophorose and glucose as the carbon source using RNA-seq approach. T. reesei (QM9414) was grown in Mandels-Andreotti medium, supplemented with 1% of cellulose, 2% of glucose or 1mM of sophorose. The cultures were incubated on an orbital shaker (200 rpm) at 28M-BM-0C for 24, 48 and 72 hours using cellulose as carbon source; for 24 and 48 hours with glucose as the carbon; and 2, 4 and 6 hours with sophorose as

ORGANISM(S): Trichoderma reesei

SUBMITTER: Gabriela Persinoti 

PROVIDER: E-GEOD-53629 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress