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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from cultured human dermal fibroblasts

ABSTRACT: We used microarrays to identify the molecular targets of a novel small compound HSc025. Fibroblasts were exposed to four different stimulants, DMSO, HSc025, TGF-bata or HSc025 plus TGF-beta.

ORGANISM(S): Homo sapiens

SUBMITTER: Kiyoshi Higashi

PROVIDER: E-GEOD-53672 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from TGF-beta-treated human ovarian fibroblasts

Project description:Advanced ovarian cancer is the most lethal gynecologic malignancy in the United States. Ovarian cancer cells are known to have diminished response to TGF-beta, but it remains unclear whether TGF-beta can modulate ovarian cancer cell growth in an indirect manner through cancer-associated fibroblasts (CAFs). Using transcriptome profiling analyses on TGF-beta-treated ovarian fibroblasts, we identified a TGF-beta-responsive gene signature in ovarian fibroblasts. Identifying TGF-beta-regulated genes in the ovarian microenvironment helps in understanding the role of TGF-beta in ovarian cancer progression. The human telomerase-immortalized ovarian fibroblast line NOF151 was treated with 5ng/mL of either TGF-beta-1 or TGF-beta-2. Total RNA was isolated from control samples and TGF-beta-treated fibroblasts samples at 48 hours post-treatment, followed by cDNA synthesis, IVT and biotin labeling. Samples were then hybridized onto Affymetrix Human Genome U133 Plus 2.0 microarrays. For each treatment group, three independent samples were prepared for the microarray experiment.

2013-12-31 | E-GEOD-40266 | biostudies-arrayexpress

Expression data from skin of mice treated subcutaneously with TGF-beta, IL-13 or TSLP for 7 days

Project description:Gene expression in mice skin stimulated with 3 different cytokines We used microarrays to detail the global programme of specific or common gene expression by 3 cytokines: TGF-beta, IL-13 and TSLP Mice skin were taken from the outlet of the 7-day pump (subcutaneous) and RNA was extracted.

2013-10-31 | E-GEOD-34297 | biostudies-arrayexpress

Transcription profiling of human pulp and odontoblasts reponse to TGF-beta1

Project description:Transforming growth factor beta 1 (TGF-Î²1) is the most extensively studied growth factor in dentin-pulp complex, with pleiotropic effects on pulp response and healing. Our main objective was to analyze the expression profile of pulp tissue and odontoblasts, and the effects of TGF-Î²1 on these profiles in cultured human pulp and odontoblasts with a specific interest in the anti- and pro-inflammatory cytokines. Experiment Overall Design: Pulp tissues and odontoblasts were cultured for different time periods, and microarray was performed to both cultured and native samples to detect the effects of TGF-Î²1. Expression of various interleukins (IL) were confirmed by RT-PCR, and in +/- TGF-Î²1 treated pulps also by antibody array.

2008-06-16 | E-GEOD-8730 | biostudies-arrayexpress

Transcription profiling of human glioma cells treated with TGF-beta or an inhibitor of the TGF-beta Receptor I LY2109761

Project description:In order to identify the genes that are regulated by TGF-beta in glioma, we serum starved two glioma cell lines, U373MG and U87MG, for 16h and we treated them with vehicle,100pM TGF-beta, 2uM inhibitor of the TGF-beta Receptor I(TbRI)(LY2109761), or both 100pM TGF-beta plus 2uM TbRI for 3h. Then, cell were collected and total RNA was extracted.

2007-05-16 | E-MEXP-903 | biostudies-arrayexpress

Transcription profiling of mouse wild type or Batf deficient T cells cultured under various combinations of IL-6 orTGF-beta

Project description:Wild type and Batf deficient T cells were stimulated under various combinations of IL-6 or TGF-beta.

2009-06-01 | E-MEXP-2152 | biostudies-arrayexpress

Transcription profiling of human thyroid papillary carcinoma cells treated with TGF-b or untreated

Project description:We used primary culture of Human thyroid papillary carcinoma to perform the experiment. We make two group one without TGF-b treatment and another with 2nanogr of TGF-b for 4 days. The RNA was extracted and the samples were processed for the array assay.<br><br><br><br>

2009-11-23 | E-MEXP-2045 | biostudies-arrayexpress

Protection from obesity and diabetes by blockade of TGF-beta/Smad3 signaling

Project description:Imbalances in glucose and energy homeostasis are at the core of the worldwide epidemic of obesity and diabetes. Here, we illustrate an important role of the TGF-beta/Smad3 signaling pathway in regulating glucose and energy homeostasis. Smad3 deficient mice are protected from diet-induced obesity and diabetes. Interestingly, the metabolic protection is accompanied by Smad3-/- white adipose tissue acquiring the bioenergetic and gene expression profile of brown fat/skeletal muscle. Smad3-/- adipocytes demonstrate a marked increase in mitochondrial biogenesis, with a corresponding increase in basal respiration, and Smad3 acts as a repressor of PGC-alpha1 expression. We observe significant correlation between TGF-beta1 levels and adiposity in rodents and humans. Further, systemic blockade of TGF-beta1 signaling protects mice from obesity, diabetes and hepatic steatosis. Together, these results demonstrate that TGF-beta signaling regulates glucose tolerance and energy homeostasis and suggest that modulation of TGF-beta1 activity might be an effective treatment strategy for obesity and diabetes. Smad3-/- and WT mice were fed with regular diet (RD) and high fat diet (HFD), and diet induced obese (DIO) mice were treated with IgG and anti-TGF-b1 antibody

2011-07-12 | E-GEOD-28598 | biostudies-arrayexpress

Olig1 is a Smad cofactor involved in cell motility induced by transforming growth factor-b

Project description:Transforming growth factor (TGF)-Î² plays crucial roles in embryonic development and adult tissue homeostasis by eliciting various cellular responses in target cells. TGF-Î² signaling is principally mediated through receptor-activated Smad proteins, which regulate expression of target genes in cooperation with other DNA-binding transcriptionfactors (Smad cofactors). In this study, we found that the basic helix-loop-helix transcription factor Olig1 is a Smad cofactor involved in TGF-b-induced cell motility. Knockdown of Olig1 attenuated TGF-Î²-induced cell motility in chamber migration and wound healing assays. In contrast, Olig1 knockdown had no effect on bone morphogenetic protein-induced cell motility, TGF-Î²-induced cytostasis or epithelial-mesenchymal transition. Furthermore, we observed that cooperation of Smad2/3 with Olig1 is regulated by a peptidyl-prolyl cis/trans isomerase, Pin1. TGF-b-induced cell motility, induction of Olig1-regulated genes, and physical interaction between Smad2/3 and Olig1 were all inhibited after knockdown of Pin1, indicating a novel mode of regulation of Smad signaling. We also found that Olig1 interacts with the L3 loop of Smad3. Using a synthetic peptide corresponding to the L3 loop of Smad3, we succeeded in selectively inhibiting TGF-b-induced cell motility. These findings may lead to a new strategy for selective regulation of TGF-b-induced cellular responses. NMuMG cells were transfected with siRNAs (siControl, siOlig1 or siPin1) and treated with or without TGF-b for 1h. We compared genes affected by knockdown of Olig1 and that of Pin1.

2013-07-01 | E-GEOD-46405 | biostudies-arrayexpress

Expression profiling in palatal mesenchymal cells stimulated with TGF-beta2 in the presence and absence of TGFbRI and Tak1 kinase inhibitors

Project description:TGF-beta signaling in neural crest cells is required for normal craniofacial development. This signaling can be transduced via TGF-beta type I receptors (TGFbRI) using Smad-dependent or Smad independent signaling pathways. We used microarrays to identify TGF-beta-responsive genes that are dependent either on TGFbRI kinase, Tak1 kinase or both. Primary palatal mesenchymal cell cultures were established. Cultured cells were stimulated with TGF-beta2 in the presence or absence of TGFbRI kinase and Tak1 kinase inhibitors. Unstimulated cells were used as controls. Total RNAs were isolated and hybridized on Affymetrix microarrays.

2013-03-27 | E-GEOD-45491 | biostudies-arrayexpress

TM4SF20 is reduced by TGF-Î² in A549 cells

Project description:To find out genes regulated by TGF-Î² in A549 cells, we compared gene expression of cells treated with 1ng/ml TGF-Î² versus non-treated cells and find out that expression of one transmembrane protein, TM4SF20, is reduced by TGF-Î². A549 cells were treated with or without 1ng/ml TGF-Î² for 12h. RNA was extracted and hybridized on Affymetrix microarrays

2014-08-04 | E-GEOD-46024 | biostudies-arrayexpress

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