Transcription profiling of human airway epithelium, large airways, pre and post-mechanical injury

ABSTRACT: Responses of the Human Airway Epithelium Transcriptome to In Vivo Injury; To identify genes participating in repair of the human airway epithelium following injury, we used bronchoscopy and brushing to denude the airway epithelium of healthy individuals, sequentially sampled the same region 7 and 14 days later, and assessed the recovered epithelium for relative levels of gene expression using Affymetrix high-density oligonucleotide microarrays with TaqMan PCR confirmation. Histologic assessment showed that the epithelium was denuded immediately following injury, at 7 days the epithelium was completely covered but partially de-differentiated, and by 14 days there was close to normal proportions of differentiated cells. Gene expression analysis was carried out with both the Affymetrix Microa

ORGANISM(S): Homo sapiens

SUBMITTER: Jacqueline Salit 

PROVIDER: E-GEOD-5372 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress