Genetic heterogeneity versus molecular analysis of prion susceptibility in neuroblasma N2a sublines

ABSTRACT: The neuroblastoma-derived cell line N2a is permissive to certain prion strains but resistant sublines unable to accumulate the pathological proteinase-K resistant form of the prion protein can be isolated. We compared for gene expression and phenotypes different N2a sublines that were susceptible or resistant to the 22L prion strain. Karyotypes and comparative genomic hybridization arrays revealed chromosomal imbalances but did not demonstrate a characteristic profile of genomic alterations linked to prion susceptibility. Likewise, we showed that this phenotype was not dependent on the binding of PrPres, the expression of the prion protein gene, or on its primary sequence. We completed this analysis by looking using real-time quantitative PCR at the expression of a set of genes encoding pr

ORGANISM(S): Mus musculus

SUBMITTER: Alexander Burkle 

PROVIDER: E-GEOD-5374 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress