Absolute quantification of protein production reveals principles underlying protein synthesis rates

ABSTRACT: A quantitative view of cellular functions requires precise measures of the rates of biomolecule production, especially proteins-the direct effectors of biological processes. Here we present a genome-wide approach, based on ribosome profiling, for measuring absolute protein synthesis rates. The resultant E. coli dataset transforms our understanding of the extent to which protein synthesis is precisely controlled to optimize function and efficiency. For example, members of multi-protein complexes are made in precise proportion to their stoichiometry, whereas components of functional modules are produced differentially according to their hierarchical role. Estimates of absolute protein abundance also reveal principles used to optimize design. These include how the level of different types of

ORGANISM(S): Escherichia coli

SUBMITTER: Gene-Wei Li 

PROVIDER: E-GEOD-53767 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress