The DEAD box helicase RDE-12 promotes amplification of RNAi in cytoplasmic foci in C. elegans

ABSTRACT: RNA interference (RNAi) is a potent mechanism for down-regulating gene expression. Conserved RNAi pathway components are found in animals, plants, fungi and other eukaryotes. In C. elegans, the RNAi response is greatly amplified by the synthesis of abundant secondary siRNAs. Exogenous double stranded RNA is processed by Dicer and RDE-1/Argonaute into primary siRNA that guides target mRNA recognition. The RDE-10/RDE-11 complex and the RNA dependent RNA polymerase RRF-1 then engage the target mRNA for secondary siRNA synthesis. However, the molecular link between primary siRNA production and secondary siRNA synthesis remains largely unknown. Furthermore, it is unclear if the sub-cellular sites for target mRNA recognition and degradation coincide with sites where siRNA synthesis and amplifi

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Huan Yang 

PROVIDER: E-GEOD-53830 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress