Medial Tibial Plateau articular cartilage changes 2 ,4 and 8 weeks after DMM

ABSTRACT: Objective: To Identify gene changes in the medial tibial plateau of articular cartilage at 2, 4 and 8 weeks after destabilisation of the medial meniscus (DMM) in mice and compare our data set with previously published sets to ascertain dysregulated pathways and genes in osteoarthritis. Materials and methods: RNA was extracted from the ipsilateral and contralateral medial tibial plateaus, amplified, labelled and hybridized on Illumina WG_v2 microarrays. Results were confirmed by RT-PCR for selected genes. Results: Transcriptional analysis and network reconstruction revealed changes in extracellular matrix (ECM) and cytoskeletal genes induced by DMM. TGFalpha signalling pathway and complement and coagulation cascade genes were regulated at 2 weeks. Fibronectin (FN1) is a hub in a reconstru

ORGANISM(S): Mus musculus

SUBMITTER: Anastasios Chanalaris 

PROVIDER: E-GEOD-53857 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress