Differentiation of human limbal-derived induced pluripotent stem cells into limbal-like epithelium

ABSTRACT: Limbal epithelial stem cell (LESC) deficiency represents a significant clinical problem especially in bilateral cases. Induced pluripotent stem cells (iPSC) may be a promising source of LESC, allowing standardized and continual propagation and banking. The objective of this study was to generate iPSC from human limbal epithelial cultures and differentiate them back into limbal epithelial cells using substrata mimicking the natural LESC niche. Using Yamanaka’s episomal vectors limbal-derived iPSC were reprogrammed from LESC cultured from donor corneoscleral rims and from human skin fibroblasts. A clone from limbal-derived iPSC expressed stemness markers, had a diploid karyotype, and produced teratomas in nude mice representing three germ layers. Compared to parental LESC, this clone had few

ORGANISM(S): Homo sapiens

SUBMITTER: Jie Tang 

PROVIDER: E-GEOD-53918 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress