TOP1M-NM-1 functions on nucleosome density

ABSTRACT: DNA topoisomerases release supercoils in DNA introduced during replication or transcription. How DNA topoisomerases impact transcription in the context of eukaryotic chromatin is poorly understood. In this study, using a floral stem cell model in Arabidopsis, we uncovered a role of TOP1M-NM-1 in Polycomb Group (PcG) protein-mediated histone lysine 27 trimethylation at, and transcriptional repression of, the stem cell maintenance gene WUSCHEL (WUS). The strong genetic interactions between a top1M-NM-1 mutant and mutations in PcG genes and the overwhelming enrichment of PcG targets among genes affected in expression in the top1a mutant revealed a role of TOP1M-NM-1 in PcG-mediated regulation. Intriguingly, not only the repression of some PcG target genes but also the expression of others req

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Lei Gao 

PROVIDER: E-GEOD-53950 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress