Expression analysis of DamP mRNAs using expressed molecular barcodes

ABSTRACT: We have generated 979 yeast strains in which the natural 3' UTR of essential gene mRNAs has been replaced by the same long 1.4 kb artificial 3' UTR (DAmP modification). Nonsense mediated mRNA decay (NMD) of these mRNA reporters was tested by using Agilent barcode microarrays by taking advantage of molecular barcodes introduced just downstream the stop codon during strain construction. We introduced in each DAmP strain either a neutral mutation (deletion of YEL068C) or the deletion of essential factors for NMD: NAM7 and NMD2. The resulting haploid cells were tested for changes in DAmP mRNA levels by comparing an RNA sample with a DNA genomic sample. Differences between the samples indicate the sensibility of the DAmP mRNAs to degradation through NMD. The large scale results were validated b

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Cosmin Saveanu 

PROVIDER: E-GEOD-53954 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress