Transcrip and physiological responses of chemostat-grown Campylobacter jejuni to S-nitrosoglutathione

ABSTRACT: A single-domain globin (cgb) mutant strain of Campylobacter jejuni NCTC11168 was grown in 2 homemade, parallel chemostats. Cells were grown in 125 ml volumes of Mueller-Hinton Broth where the growth rate was controlled by the dilution rate (0.1 h-1). A gas mix of 80% nitrogen, 10% carbon dioxide and 10% oxygen was pumped into the head-space of the vessels at a rate of 0.25 l/min to obtain a microaerobic environment and the culture was maintained at 42 ºC via a water jacket. Cells were grown as above until steady-state had been reached. At steady-state one of the cultures was exposed to 0.25 mM GSNO (final concentration) for a period of 10 min. Samples of both the control and stressed cultures were harvested into phenol/ethanol to stabilize the RNA and total RNA was purified using Qiagenâ

ORGANISM(S): Campylobacter jejuni

SUBMITTER: Claire Monk 

PROVIDER: E-GEOD-5396 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress