Estrogen Receptor binding site mapping by ChIP-on-chip

ABSTRACT: MCF-7 breast cancer cells were treated with estradiol and then chromatin immunoprecipitation assays were performed to isolate estrogen receptor and associated genomic DNA binding sites. ChIP DNA fragments were end-labeled with biotinylated nucleotides and then labeled with Cy3-avidin and hybridized to human promoter arrays to detect estrogen receptor binding sites.

ORGANISM(S): Homo sapiens

SUBMITTER: Chin-Yo Lin 

PROVIDER: E-GEOD-5405 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress