Human Hepatocytes with Drug Metabolic Function Induced from Fibroblasts by Lineage Reprogramming
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ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Jinlin Wang
PROVIDER: E-GEOD-54066 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Human hepatocytes with drug metabolic function induced from fibroblasts by lineage reprogramming.
Du Yuanyuan Y Wang Jinlin J Jia Jun J Song Nan N Xiang Chengang C Xu Jun J Hou Zhiyuan Z Su Xiaohua X Liu Bei B Jiang Tao T Zhao Dongxin D Sun Yingli Y Shu Jian J Guo Qingliang Q Yin Ming M Sun Da D Lu Shichun S Shi Yan Y Deng Hongkui H
Cell stem cell 20140227 3
Obtaining fully functional cell types is a major challenge for drug discovery and regenerative medicine. Currently, a fundamental solution to this key problem is still lacking. Here, we show that functional human induced hepatocytes (hiHeps) can be generated from fibroblasts by overexpressing the hepatic fate conversion factors HNF1A, HNF4A, and HNF6 along with the maturation factors ATF5, PROX1, and CEBPA. hiHeps express a spectrum of phase I and II drug-metabolizing enzymes and phase III drug ...[more]
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