Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Auratus goldfish genome project: effects of fluoxetine on female goldfish

ABSTRACT: A goldfish brain enriched cDNA combined with a carp microarray (Gracey et al., 2004). Female adult goldish were treated twice a week for 17 days, for a total of five injections. Each of the injections consisted of 5 μg fluoxetine/g of body weight with an injection volume of 1μl/g. The telencephalon was tissue used. Keywords: i.p.injection of muscimol, 6 hours, pharmacological 4 slides (n=3 treatment samples and common control pool) slide 19 = treatment sample 1 slide 30 = treatment sample 2 slide 36 = treatment sample 3 (dye swap) slide 39 = treatment sample 3 (dye swap)

ORGANISM(S): Carassius auratus

SUBMITTER: Jan Mennigen 

PROVIDER: E-GEOD-5420 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Effects of fluoxetine on the reproductive axis of female goldfish (Carassius auratus).

Mennigen Jan A JA   Martyniuk Christopher J CJ   Crump Kate K   Xiong Huiling H   Zhao E E   Popesku Jason J   Anisman Hymie H   Cossins Andrew R AR   Xia Xuhua X   Trudeau Vance L VL  

Physiological genomics 20080902 3

We investigated the effects of fluoxetine, a selective serotonin reuptake inhibitor, on neuroendocrine function and the reproductive axis in female goldfish. Fish were given intraperitoneal injections of fluoxetine twice a week for 14 days, resulting in five injections of 5 microg fluoxetine/g body wt. We measured the monoamine neurotransmitters serotonin, dopamine, and norepinephrine in addition to their metabolites with HPLC. Homovanillic acid, a metabolite in the dopaminergic pathway, increas  ...[more]

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