Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from Saccharomyces cerevisiae strains carrying the spt16-11 or the pob3-7 allele

ABSTRACT: The conserved FACT (FAcilitates Chromatin Transcription) complex is a chromatin-reorganizing complex that promotes RNAPII transcription through chromatin templates by interacting with histones. It facilitates promoter activation by nucleosome eviction, and transcription elongation by nucleosome disruption and reassembly ahead and behind the RNAP. It also has a role in replication not fully understood yet. Genome-wide microarray analyses in spt16-11 and pob3-7 strains revealed a set of genes whose mRNA levels were altered with respect to the WT levels. These include 48 up-regulated and 80 down-regulated genes that are common to both strains. The up-regulated genes were longer and expressed at lower levels than the genome average whereas the down-regulated genes were more similar to the average of the genome. S. cerevisiae strains were grown in YEPD liquid culture 4 h after a 26-to-30ºC temperature shift, total RNA was isolated and hybridized on Affymetrix microarrays.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Andrés Aguilera 

PROVIDER: E-GEOD-54340 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The yeast and human FACT chromatin-reorganizing complexes solve R-loop-mediated transcription-replication conflicts.

Herrera-Moyano Emilia E   Mergui Xénia X   García-Rubio María L ML   Barroso Sonia S   Aguilera Andrés A  

Genes & development 20140317 7

FACT (facilitates chromatin transcription) is a chromatin-reorganizing complex that swaps nucleosomes around the RNA polymerase during transcription elongation and has a role in replication that is not fully understood yet. Here we show that recombination factors are required for the survival of yeast FACT mutants, consistent with an accumulation of DNA breaks that we detected by Rad52 foci and transcription-dependent hyperrecombination. Breaks also accumulate in FACT-depleted human cells, as sh  ...[more]

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