Transcriptome analysis of human umbilical cord fibroblasts from babies whose mother experienced preeclampsia

ABSTRACT: In pregnancies involving preeclampsia (PE), there is evidence that the fetal-placental unit is under oxidative stress. Here we examined primary cell lines generated from umbilical cords (UC) delivered by mothers who had either a normal pregnancy or experienced early onset PE to determine whether the two had distinguishable phenotypes. While all UC provided outgrowths when established in 4 % O2, success was less assured for PE cords under ambient (20 % O2) conditions (P < 0.05). Moreover, proliferation rates of established PE lines, although similar to controls in 4 % O2, were significantly lower in 20 % O2. PE lines grown in 4 % O2 were also more susceptible to the pro-oxidant diethylmaleate than control lines, and unlike controls, were not protected by glutathione. Transcriptome profilin

ORGANISM(S): Homo sapiens

SUBMITTER: Toshihiko Ezashi 

PROVIDER: E-GEOD-54400 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress