Comparison of exosomal and cellular miRNA from patients with chronic lymphocytic leukaemia

ABSTRACT: Exosomes are secreted into the intercellular space and carry a diverse cargo of proteins, mRNA and miRNA that have important roles in cell-to-cell communication. We found that exosomes from primary human leukaemic B-cells contain a population of miRNAs that has been selected from the general cellular pool to be enriched in miR-323-3p, miR-374b, miR-363 and miR-494. Gene targets of these miRNA are found within the TGFM-NM-2 pathway, but we noted that CD69, a protein that is essential for the regulation of normal lymphocyte egress from lymph nodes was the top predicted target of miR-363. Utilising luciferase reporter assays we demonstrate that CD69 is a target of miR-363. The interaction between miR-363 and CD69 is functionally important as demonstrated by repression of CD69 expression by a

ORGANISM(S): Homo sapiens

SUBMITTER: Simon Wagner 

PROVIDER: E-GEOD-54434 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress