Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Downregulation of micro RNAs 145-3p and 145-5p is a Long-Term Predictor of Postmenopausal Breast Cancer Risk: the ORDET prospective study

ABSTRACT: microRNA expression profile of buffy coat derived from healty woman before the insorgence of breast cancer microRNA expression was evaluated from total RNA extracted from buffy coat.

ORGANISM(S): Homo sapiens

SUBMITTER: andrea sacconi

PROVIDER: E-GEOD-54470 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Downregulation of microRNAs 145-3p and 145-5p is a long-term predictor of postmenopausal breast cancer risk: The ORDET prospective study.

Muti Paola P Sacconi Andrea A Hossain Ahmed A Donzelli Sara S Ben Moshe Noa Bossel NB Ganci Federica F Sieri Sabina S Krogh Vittorio V Berrino Franco F Biagioni Francesca F Strano Sabrina S Beyene Joseph J Yarden Yosef Y Blandino Giovanni G

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 20140729 11

<h4>Background</h4>miRNAs have been implicated in the regulation of key metabolic, inflammatory, and malignant pathways; hence, they might be considered both predictors and players of cancer development.<h4>Methods</h4>Using a case-control study design nested in the ORDET prospective cohort study, we addressed the possibility that specific mRNAs can serve as early predictors of breast cancer incidence in postmenopausal women. We compared leukocyte miRNA profiles of 133 incident postmenopausal br ...[more]

PMID: 25073626

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Project description:Purpose: There is a quest for novel non-invasive diagnostic markers for the detection of breast cancer. The goal of this study is to identify circulating microRNA signatures using a cohort of Asian Chinese breast cancer patients, and to compare microRNA profiles between tumour and serum samples. Experimental design: MicroRNAs from paired breast cancer tumours, normal tissue and serum samples derived from 32 patients were comprehensively profiled using microarrays (1300 microRNAs against tumour and normal tissues) or LNA RT-PCR panels (742 microRNAs against serum samples). Serum samples from healthy individuals (n=22) were also employed as normal controls. Significant serum microRNAs, identified by logistic regression, were validated in an independent set of serum samples from patients (n=82) and healthy controls (n=53). Results: The 20 most significant microRNAs differentially expressed in breast cancer tumours included miR-21, miR-10b, and miR-145, previously shown to be dysregulated in breast cancer. Interestingly, 16 of the 20 most significant microRNAs differentially expressed in serum samples were novel. MiR-1, miR-92a, miR-133a and miR-133b were identified as the most important diagnostic markers, and were successfully validated; receiver operating characteristic curves derived from combinations of these microRNAs exhibited areas under the curves of 0.944-0.946. Only seven microRNAs were overexpressed in both tumours and serum, suggesting that microRNAs may be released into the serum selectively. Conclusion: The clinical employment of microRNA signatures as a non-invasive diagnostic strategy is promising, but should be further validated for different subtypes of breast cancers. "_A" and "_B" are two tissue sections of the same sample; "_1" and "_2" represents 2 runs of the same sample; na = not available All tissue samples were histologically confirmed by a pathologist using hematoxylin and eosin staining of cryosectioned specimens. One tumour sample was rejected due to failure to detect any tumour cells. Except for two samples (with 30% and 40% tumour cells), all tumour tissues employed had a minimum of 60% tumour cells, as estimated microscopically. Overall, the breast cancer tumour samples had an average of 71% tumour cells. The criteria for adjacent normal tissue were absence of tumour cells and presence of epithelial cells. Hence, after histological confirmation, 31 breast cancer tumours and 23 matched normal tissues were employed for microRNA extraction and profiling using microarray.

Dataset Information

Downregulation of micro RNAs 145-3p and 145-5p is a Long-Term Predictor of Postmenopausal Breast Cancer Risk: the ORDET prospective study

Publications

Downregulation of microRNAs 145-3p and 145-5p is a long-term predictor of postmenopausal breast cancer risk: The ORDET prospective study.

Similar Datasets

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