Selective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP

ABSTRACT: The nuclear phase of the gene expression pathway culminates in the export of mature mRNAs to the cytoplasm through nuclear pore complexes (NPCs). GANP (Germinal-centre Associated Nuclear Protein) promotes the transfer to NPCs of mRNAs bound to the transport factor NXF1. Here, we demonstrate that GANP, subunit of the TREX-2 mRNA export complex, promotes selective nuclear export of a specific subset of mRNAs whose transport depends on NXF1. Genome-wide gene expression profiling showed that half of the transcripts whose nuclear export was impaired following NXF1 depletion also showed reduced export when GANP was depleted. GANP-dependent transcripts were highly expressed, yet short-lived, and were highly enriched in those encoding central components of the gene expression machinery such as RNA

ORGANISM(S): Homo sapiens

SUBMITTER: Vihandha Wickramasinghe 

PROVIDER: E-GEOD-54481 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress