Transcription profiling of mouse brains of E14.5, E16.5 and E18.5 Top2b+/+ and top2b-/- embryos to study the role of DNA Topoisomerase IIbeta in gene expression

ABSTRACT: Mice lacking topoisomerase IIbeta (Top IIbeta) are known to exhibit a perinatal death phenotype. In the current study, transcription profiles of the brains of wild type and top2beta knockout mouse embryos were generated. Surprisingly, only a small number (1-4%) of genes were affected in top2beta knockout embryos. However, the expression of nearly 30% of developmentally regulated genes was either up- or down-regulated. By contrast, the expression of genes encoding general cell growth functions and early differentiation markers was not affected, suggesting that TopIIbeta is not required for early differentiation programming, but is specifically required for the expression of developmentally regulated genes at later stages of differentiation. Consistent with this notion, immunohistochemical a

ORGANISM(S): Mus musculus

SUBMITTER: Yi Lisa Lyu 

PROVIDER: E-GEOD-5458 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress