Differential in vivo tumorigenicity of distinct subpopulations from a luminal-like breast cancer model (SNP)

ABSTRACT: The cellular heterogeneity of one patient derived orthotopic breast cancer xenograft model PDX was investigated using flow cytometry, combined with assessment of in vivo tumorigenicity and SNP genotyping array for copy number analysis. Epithelial cell adhesion molecule (EpCAM) was revealed as a highly specific cell surface marker of the human tumor cell population in both xenografts. Based on expression patterns observed in primary tumor tissue, SSEA-4 and CD24 were chosen as markers to further subdivide the luminal tumor cells into four subpopulations. FACS sorting was used to isolate four cell subpopulations. Results: In vivo tumorigenicity assay showed that SSEA-4+/CD24+ cells were non-tumorigenic, while the three other subpopulations were tumorigenic. Tumors resulting from the SSEA-4

ORGANISM(S): Homo sapiens

SUBMITTER: Nirma Skrbo 

PROVIDER: E-GEOD-54595 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress