Next Generation Sequencing facilitates analysis of gene expression from Rehmannia glutinosa associated with replanting disease

ABSTRACT: Purpose: the technology of Solexa/Illumina (RNA-seq) is an attractive alternative to the transcriptome sequencing. The goals of this study are to investigate transcriptional changes in replanted R. glutinosa leaves and identify genes responding to the disease. Methods:Totally 6.01and 6.15 million raw tags were measured from leaves of the first year planted (L1) and the second year replanted R. glutinosa (L2) at the tuberous root expansion stage, respectively. mRNA profiles of leaves of the first year planted (L1) and the second year replanted R. glutinosa (L2) at the tuberous root expansion stage, were generated by deep sequencing (Illumina HiSeq™ 2000 system). qRT–PCR validation was performed using SYBR Green assays. Results: we obtained a total of 6.01 and 6.15 million total tags

ORGANISM(S): Rehmannia glutinosa

SUBMITTER: Xin Jian Chen 

PROVIDER: E-GEOD-54620 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress