Host cell responses to persistent mycoplasmas--different stages in infection of HeLa cells with Mycoplasma hominis

ABSTRACT: M. hominis adherence, colonisation and invasion of HeLa cells were characterised in a time-course study using microarray-based analysis of the HeLa cell transcriptome: 0 h to monitor the baseline of transcription, 4 h to examine host reactions to mycoplasma attachment, 48 h to capture in addition the initiation of invasion, and 2 weeks post infection to examine a chronically infected host cell. At 4 h post infection, cytoadherence of M. hominis to the HeLa cell surface was accompanied by differential regulation of 723 host genes (>2 fold change in expression). Genes associated with immune responses and signal transduction pathways were mainly affected and components involved in cell-cycle regulation, growth and death were highly upregulated. At 48 h post infection, when mycoplasma invasion

ORGANISM(S): Homo sapiens

SUBMITTER: Rene Deenen 

PROVIDER: E-GEOD-54698 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress