Quality assessment and control of tissue specific RNA-seq libraries of Drosophila transgenic RNAi models

ABSTRACT: Next-generation RNA-sequencing (RNA-seq) is rapidly emerging as the technology of choice for whole-transcriptome studies. However, RNA-seq is not a bias free technique. It requires large amounts of RNA and library preparation can introduce multiple artifacts, compounded by problems from later stages in the process. Nevertheless, RNA-seq is increasingly used in multiple studies, including the characterization of tissue-specific transcriptomes from invertebrate models of human disease. The generation of samples in this context is complex, involving the establishment of mutant strains and the delicate contamination prone process of dissecting the target tissue. Moreover, in order achieve the required amount of RNA, multiple samples need to be pooled. Such datasets pose extra challenges due to

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Andreia Amaral 

PROVIDER: E-GEOD-54724 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress