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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human HMEC after stimulation with MRP8/MRP14

ABSTRACT: GEnerating the the gene expression profil of MRP8/MRP14-stimulated HMEC monolayer. Experiment Overall Design: 4 independent experiments Experiment Overall Design: HMEC-1 monolayer were left untreated or stimulated for 6h with 200 Âµg/ml MRP8/MRP14.

ORGANISM(S): Homo sapiens

SUBMITTER: Dorothee Viemann

PROVIDER: E-GEOD-5474 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MRP8/MRP14 impairs endothelial integrity and induces a caspase-dependent and -independent cell death program.

Viemann Dorothee D Barczyk Katarzyna K Vogl Thomas T Fischer Ute U Sunderkötter Cord C Schulze-Osthoff Klaus K Roth Johannes J

Blood 20061109 6

Activated phagocytes express considerable amounts of MRP8 and MRP14, 2 calcium-binding S100 proteins forming stable heterodimers that are specifically secreted at inflammatory sites in many diseases. We previously reported that treatment of human microvascular endothelial cells with purified MRP8/MRP14 leads to loss of endothelial cell contacts. In this study, we demonstrate that MRP8/MRP14 complexes furthermore trigger cell death of endothelial cells after the onset of cell detachment. Morpholo ...[more]

PMID: 17095618

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Genome-wide expression analysis demonstrates a dominant role of TLR4 for activation of human phagocytes by the alarmin MRP8

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