Genome-wide replication analysis of a yeast eco1 mutant by deep sequencing.

ABSTRACT: Eco1 is the acetyltransferase that establishes sister-chromatid cohesion during DNA replication. Budding yeast with an eco1 mutation that genocopies Roberts syndrome displaysreduced ribosomal DNA (rDNA) transcription and a transcriptional signature of starvation. Weshow that deleting FOB1, a gene encoding a specific replication fork blocking protein for therDNA region, rescues rRNA production and partially rescues transcription genome-wide. This experiment examines the effect of eco1 mutation on replication genome-wide. Furtherstudies show that deletion of FOB1 corrects the genome-wide replication defects, nucleolarstructure, and rDNA segregation in an eco1 mutant. Our study highlights cohesin's central role atthe rDNA for global control of DNA replication and gene expression. DNA content

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Chris Seidel 

PROVIDER: E-GEOD-54742 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress