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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome analyses of the Laccaria bicolor-Populus trichocarpa mycorrhiza development

ABSTRACT: Illumina GAIIx technology was used to generate mRNA profiles from the ectomycorrhizal fungi Laccaria bicolor colonizing roots of Populus trichocarpa. Samples were taken after two, four and 12 weeks of contact in order to identify mycorrhiza-regulated transcripts. 37bp reads were generated and aligned to the Populus trichocarpa (http://www.phytozome.net/poplar.php) and the Laccaria bicolor (http://genome.jgi-psf.org/Lacbi2/Lacbi2.home.html) reference genomes using CLC Genomics Workbench 6. mRNA profiles from Populus trichocarpa roots colonized by Laccaria bicolor for two, four and 12 weeks as well as from control roots and free-living mycelium were generated by using one lane of 37 bp Illumina GAIIx sequencing per sample.

ORGANISM(S): Populus trichocarpa

SUBMITTER: Annegret Kohler

PROVIDER: E-GEOD-54789 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Populus trichocarpa and Populus deltoides exhibit different metabolomic responses to colonization by the symbiotic fungus Laccaria bicolor.

Tschaplinski Timothy J TJ Plett Jonathan M JM Engle Nancy L NL Deveau Aurelie A Cushman Katherine C KC Martin Madhavi Z MZ Doktycz Mitchel J MJ Tuskan Gerald A GA Brun Annick A Kohler Annegret A Martin Francis F

Molecular plant-microbe interactions : MPMI 20140601 6

Within boreal and temperate forest ecosystems, the majority of trees and shrubs form beneficial relationships with mutualistic ectomycorrhizal (ECM) fungi that support plant health through increased access to nutrients as well as aiding in stress and pest tolerance. The intimate interaction between fungal hyphae and plant roots results in a new symbiotic "organ" called the ECM root tip. Little is understood concerning the metabolic reprogramming that favors the formation of this hybrid tissue in ...[more]

PMID: 24548064

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