Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Comparison of gene expression pattern between Wild-type and Setd2 knockout mESCs [Affymetrix]

ABSTRACT: SETD2/HYPB has been known as a histone H3K36 specific methyltransferase. However, its roles in physiology such as development and cellular function remain unclear. In this study, using mESCs as cellular model, we show that Setd2 mainly regulates differentiation of murine embryonic stem cells (mESCs) towards primitive endoderm. This study aimed at exploring how did Setd2 regulate primitive endoderm. differentiation. We used microarrays to detail the global programme of gene expression controled by setd2, which is required for endoderm differentiation. Wild type and Setd2 knockout mESCs were selected for RNA extraction and hybridization on Affymetrix GeneChip® mouse genome 430 2.0 arrays. We sought to obtain some deregulated genes, which were required for primitive endoderm differentiation. For comparison, three biological repeats of each were performed.

ORGANISM(S): Mus musculus

SUBMITTER: Qiuhua Huang 

PROVIDER: E-GEOD-54931 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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H3K36 histone methyltransferase Setd2 is required for murine embryonic stem cell differentiation toward endoderm.

Zhang Yuanliang Y   Xie Shugao S   Zhou Yan Y   Xie Yinyin Y   Liu Ping P   Sun Mingming M   Xiao Huasheng H   Jin Ying Y   Sun Xiaojian X   Chen Zhu Z   Huang Qiuhua Q   Chen Saijuan S  

Cell reports 20140918 6

Setd2 is known as a histone-H3K36-specific methyltransferase. However, its role in physiological function remains unclear. In this study, we show that Setd2 mainly regulates differentiation of murine embryonic stem cells (mESCs) toward primitive endoderm. Furthermore, we show that downregulated endoderm-related genes in Setd2(-/-) mESCs are associated with an aberrantly low level of Erk activity and that enforced expression of Fgfr3 can rescue the defective Erk pathway in Setd2(-/-) mESCs. Inter  ...[more]

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