Analysis of sigma factor-dependent gene expression in Pseudomonas aeruginosa by RNA sequencing

ABSTRACT: Sigma factors are master regulators of bacterial transcription which direct gene expression of specific subsets of genes. In particular, alternative sigma factors are well-known to be key players of bacterial adaptation to changing environments. To elucidate the regulatory network of sigma factors in P. aeruginosa, an integrative approach including sigma factor-dependent mRNA profiling was performed to define the primary regulon of each sigma factor. Sigma factor hyper-expressing strains harboring the sigma factor gene in trans under control of the araBAD promoter and sigma factor deletion mutants were constructed. Under optimal conditions regarding sigma factor activity and optional induction of sigma factor expression, bacteria were harvested and total RNA was extracted. Upon mRNA enrich

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: Denitsa Eckweiler 

PROVIDER: E-GEOD-54998 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress