Inadequate mito-biogenesis in human dermal fibroblasts aged in situ is associated with impaired AMPK phosphorylation

ABSTRACT: Extrinsic skin ageing converges on the dermis, a post-mitotic tissue compartment consisting of extracellular matrix and long-lived fibroblasts prone to damage accumulation and maladaptation. Aged human fibroblasts exhibit mitochondrial and nuclear dysfunctions, but it is unclear whether these are cause or consequence of ageing. We report on a systematic study of human dermal fibroblasts retrieved from female donors aged 20-67 years and analyzed in primary culture at low population doubling precluding replicative senescence. Genome-wide array analysis failed to detect significant (>2-fold) age-related expression changes for individual genes, but gene set enrichment analysis revealed down regulation of many genes involved in mitochondrial metabolism and respiratory electron transport, extrac

ORGANISM(S): Homo sapiens

SUBMITTER: Hauke Busch 

PROVIDER: E-GEOD-55118 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress