Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Developmental Profile of DNA Methylation in the Developing Heart

ABSTRACT: Background: To better understand the role DNA methylation plays in regulating gene expression in the developng heart and furthermore the role it plays in heart development we performed genome wide DNA methylation profiling of embryonic hearts at embryonic day (E)11.5 and E14.5 using methyl sensitive tiny fragment enrichment coupled with massive parallel sequencing by using the methyl-sensitive restriction enzyme HpyCH4IV, recognition site 'ACGT'. Results: We found that global methylation remains stable at analyzed 'ACGT' sites (1.64 million site) in developing hearts between E11.5 and E14.5. However, differential methylation was identified at individual loci enriched at genes involved in heart development suggesting a role for DNA methylation in the developing heart. Used Methyl Sensitive Tiny Fragment Enrichment/Massive Parallel Sequencing (MSFE/MPS) to assay methylation at 'ACGT' sites throughout the genome and generate a developmental profile of DNA methylation in the embryonic heart and to identify differences between developing mouse hearts at E11.5 and E14.5.

ORGANISM(S): Mus musculus

SUBMITTER: mingyan lin 

PROVIDER: E-GEOD-55141 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DNA methylation is developmentally regulated for genes essential for cardiogenesis.

Chamberlain Alyssa A AA   Lin Mingyan M   Lister Rolanda L RL   Maslov Alex A AA   Wang Yidong Y   Suzuki Masako M   Wu Bingruo B   Greally John M JM   Zheng Deyou D   Zhou Bin B  

Journal of the American Heart Association 20140619 3

<h4>Background</h4>DNA methylation is a major epigenetic mechanism altering gene expression in development and disease. However, its role in the regulation of gene expression during heart development is incompletely understood. The aim of this study is to reveal DNA methylation in mouse embryonic hearts and its role in regulating gene expression during heart development.<h4>Methods and results</h4>We performed the genome-wide DNA methylation profiling of mouse embryonic hearts using methyl-sensi  ...[more]

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