Disomes and Disomes ubp6∆ vs. wild-type
Ontology highlight
ABSTRACT:
ORGANISM(S): Saccharomyces cerevisiae W303
SUBMITTER: Eduardo Torres
PROVIDER: E-GEOD-55166 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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2007-05-17 | E-GEOD-7812 | biostudies-arrayexpress
Project description:We constructed a database containing 248,000 exon sequences from 23,000 human RefSeq genes and compared each human exon with its best matching sequence in the January 2005 version of the rhesus genome project list of 486,000 DNA contigs. Best matching rhesus exon sequences for each of the 23,000 human genes were then concatenated in the proper order and orientation to produce a rhesus virtual transcriptome. Microarray probes were designed, one per gene, to the region closest to the 3? untranslated region (UTR) of each rhesus virtual transcript. Each probe was compared to a composite rhesus/human transcript database to test for cross-hybridization potential yielding a final probe set representing 18,296 rhesus/human gene orthologs, including transcript variants, and over 17,000 distinct genes. We hybridized mRNA from rhesus brain and spleen to both the EST- and genome-derived microarrays. Besides four-fold greater gene coverage, the genome-derived array also showed greater mean signal intensities for genes present on both arrays. Genome-derived probes showed 99.4% identity when compared to 4,767 rhesus GenBank sequence tag site (STS) sequences indicating that early stage low-pass versions of complex genomes are of sufficient quality to yield valuable functional genomic information when combined with finished genome information from a closely related species.
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2012-05-17 | E-GEOD-38016 | biostudies-arrayexpress
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