Partial phenotypic rescue of the Sesb1 mitochondrial ANT1 disease model in Drosophila

ABSTRACT: Study of gene expression patterns of Drosophila melanogaster Sesb1 mutants compared to wild type Inbreeding under stressful conditions inevitably results in the selection of compensatory alleles of many genes. In order to avoid such issues, and thus determine the global effects on gene expression of the Sesb1 mutation in a truly unselected and 'wild-type' background, we outbred Sesb1 over more than 10 generations by back-crossing to each of two commonly used wild-type strains, Canton S and Oregon R. Subsequent to this backcrossing, Sesb1 was maintained in each background using a balancer chromosome. These stocks were then used to generate a Sesb1 mutant F1 generation, by crossing virgin Canton S Sesb1. homozygous mutant females with Oregon R Sesb1 males. For comparison, we generated other

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Suvi Vartiainen 

PROVIDER: E-GEOD-55187 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress