SiRNA profiling of human H157 non-small cell lung carcinoma cells with and without DJ-1 expression knocked-down

ABSTRACT: DJ-1 expression protects H157 cells from cell death, using siRNA oligomers we knocked down DJ-1 and evaulated gene expression using Affymetrix U133A GeneChip arrays. Experiment Overall Design: H157 cells were transfected every 24 hours with siRNA oligomers for 3 consecutive days, lysed 96 hours after the first transfection, and total RNA extracted. cRNA was generated and used to probe Affymetrix U133A GeneChip microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Casey Clements 

PROVIDER: E-GEOD-5519 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress