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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse dorsal aorta derived mesangioblast clone comparison

ABSTRACT: Mouse dorsal aorta from E9.5 mouse embryos (C57BL6) was grown as an explant culture as described elsewhere (De Angelis et al., 1999). After 5 days the explant was dissociated into a single cell suspension, and plated at limiting dilution on a feeder layer of Mitomycin C-treated primary embryonic mouse fibroblasts, in 96 multiwell plates in complete medium, RPMI 20% Fetal Calf Serum. After 1 week, mesangioblast clones appeared approximately 2-4% of the wells. When the clones had grown to approximately 103 cells they were passed on gelatin-coated dishes, and further expanded. Clones D16 and D351 were used for microarray analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Enrico Tagliafico

PROVIDER: E-GEOD-552 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Msx2 and necdin combined activities are required for smooth muscle differentiation in mesoangioblast stem cells.

Brunelli Silvia S Tagliafico Enrico E De Angelis Fernanda G FG Tonlorenzi Rossana R Baesso Silvia S Ferrari Sergio S Niinobe Michio M Yoshikawa Kazuaki K Schwartz Robert J RJ Bozzoni Irene I Ferrari Stefano S Cossu Giulio G

Circulation research 20040520 12

Little is known about the molecular mechanism underlying specification and differentiation of smooth muscle (SM), and this is, at least in part, because of the few cellular systems available to study the acquisition of a SM phenotype in vitro. Mesoangioblasts are vessel-derived stem cells that can be induced to differentiate into different cell types of the mesoderm, including SM. We performed a DNA microarray analysis of a mesoangioblast clone that spontaneously expresses an immature SM phenoty ...[more]

PMID: 15155529

Similar Datasets

Mesangioblast clone comparison

Project description:Mouse dorsal aorta from E9.5 mouse embryos (C57BL6) was grown as an explant culture as described elsewhere (De Angelis et al., 1999). After 5 days the explant was dissociated into a single cell suspension, and plated at limiting dilution on a feeder layer of Mitomycin C-treated primary embryonic mouse fibroblasts, in 96 multiwell plates in complete medium, RPMI 20% Fetal Calf Serum. After 1 week, mesangioblast clones appeared approximately 2-4% of the wells. When the clones had grown to approximately 103 cells they were passed on gelatin-coated dishes, and further expanded. Clones D16 and D351 were used for microarray analysis. Keywords: other

2003-12-25 | GSE552 | GEO

Mesangioblast clone comparison- U74B

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Mesangioblast clone comparison- U74A

2003-12-25 | GSE563 | GEO

Mesangioblast clone comparison- U74C

2003-12-25 | GSE555 | GEO

Transcription profiling of mouse dorsal aorta derived mesangioblast clone comparison- U74B

2008-06-13 | E-GEOD-554 | biostudies-arrayexpress

Transcription profiling of mouse dorsal aorta from E9.5 mouse embryos (C57BL6) derived mesangioblast clone comparison

2008-06-13 | E-GEOD-555 | biostudies-arrayexpress

Transcription profiling of mouse Mouse dorsal aorta from E9.5 mouse embryos (C57BL6) explant culture for mesangioblast clone comparison- U74A

2008-06-13 | E-GEOD-563 | biostudies-arrayexpress

RNA-Seq of Metastatic Clone versus Non-Metastatic Clone in Sarcoma

Project description:We report that metastasis in an autochthonous mouse model of sarcoma is driven by a single clone in the primary tumor. We performed RNA-seq comparing the gene expression profiles of the metastatic clones (MC) to matched non-metastatic clones (non-MC) from the same tumor for multiple tumors. RNA from lung metastases (Lung-Met) of matched tumors are sequenced as well.

2019-09-03 | GSE118489 | GEO

Transcriptome analysis of the differentiated C2C12 cells in 3 different substrate, Tissue culture plate, Methacarylated gelatin and 15% Methacarylated gelatin mixed with 1% PSS (poly sodium 4-styrenesulfonate)

Project description:Purpose : To determine transcriptome profile of the diffferentiated C2C12 cells in 3 different cell culture substrate, Tissue culture plate, Methacarylated gelatin and 15% Methacarylated gelatin mixed with 1% PSS (poly sodium 4-styrenesulfonate) Method : RNA smples prepared from differentiated C2C12 cells in 3 different cell culture substrate, Tissue culture plate, Methacarylated gelatin and 15% Methacarylated gelatin mixed with 1% PSS (poly sodium 4-styrenesulfonate) Results : Paired-ends 101bp reads, we mapped about 72 million reads - 145 million reads per sample to the mouse genome (build mm10) and assembled with cufflinks program (value : FPKM)

2021-01-26 | GSE165480 | GEO

Expression data from cytotoxic T cell clone

Project description:T cells contribute to host-tumor interactions in patients with monoclonal gammopathies. Expansions of CD8+CD57+TCRVβ+ restricted cytotoxic T cell (CTL) clones are found in 48% of patients with multiple myeloma and confer a favorable prognosis. We now report the presence of CTL clones with varying TCRVβ repertoire in 70% of patients with Waldenstrom’s Macroglobulinaemia (WM) (n=20). Previous nucleoside analogue (NA) therapy, associated with an increased incidence of transformation to aggressive lymphoma, significantly influenced the presence of TCRVβ expansions (X2=11.6; P<0.001) as 83% of patients without (n=6) and only 7% with TCRVβ expansions (n=14) had received NA. Clonality of CD3+CD8+CD57+TCRVβ+ restricted CTLs were confirmed by TCRVβ CDR3 size analysis and direct sequencing. To characterize CTL clones, samples of CD3+CD8+CD57+TCRVβ+ cells were profiled using DNA microarrays and the results were validated on both gene and protein level. By gene set enrichment analysis, CTL clones not only expressed genes (GZMB, PRF1, FGFBP2) from cytotoxic pathways but also genes which suppress apoptosis, inhibit proliferation, arrest cell cycle G1/S transition and activate T cells (RAS, CSK and TOB pathways). Proliferation tracking confirmed their anergic state. Our studies demonstrate the incidence, NA sensitivity and anergic nature of clonal T cells in a B cell tumor. We used microarrays to detail the global programme of gene expression underlying T cell clonal expansion and identified distinct classes of up-regulated genes during this process.

2009-11-29 | GSE18313 | GEO

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