Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Gene expression analysis of Cbfb-deficient LSK and GMP

ABSTRACT: Runx/Cbfb heterodimers play important roles in the development of hematopoietic cells in mouse embryos and adults. In order to identify genes that are regulated by Runx/Cbfb, we purified Lin– c-kit+ Sca1+ (LSK) cells and Lin– c-kit+ Sca1– CD16/32+ (GMP) cells from Vav1-iCre x Cbfb(F/F) and Vav1-iCre x Cbfb(F/+) mice and profiled gene expression using microarray. 200,000 LSK and GMP cells were purified separately from two 7 week old Vav1-iCre x Cbfb(F/F) mice and two Vav1-iCre x Cbfb(F/+) mice by cell sorting. The purity was higher than 98%. Total RNA was extracted using the NucleoSpin RNA XS kit (Macherey-Nagel), amplified using a PicoSL RNA amplification kit (Nugen) and biotinylated with Encore biotin module (Nugen). Labeled RNA was hybridized to Mouse Gene 1.0ST microarrays (Affymetrix) according to the manufacturer’s instruction.

ORGANISM(S): Mus musculus

SUBMITTER: Takeshi Egawa 

PROVIDER: E-GEOD-55227 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Runx1 and Cbfβ regulate the development of Flt3+ dendritic cell progenitors and restrict myeloproliferative disorder.

Satpathy Ansuman T AT   Briseño Carlos G CG   Cai Xiongwei X   Michael Drew G DG   Chou Chun C   Hsiung Sunnie S   Bhattacharya Deepta D   Speck Nancy A NA   Egawa Takeshi T  

Blood 20140327 19

Runx1 and Cbfβ are critical for the establishment of definitive hematopoiesis and are implicated in leukemic transformation. Despite the absolute requirements for these factors in the development of hematopoietic stem cells and lymphocytes, their roles in the development of bone marrow progenitor subsets have not been defined. Here, we demonstrate that Cbfβ is essential for the development of Flt3(+) macrophage-dendritic cell (DC) progenitors in the bone marrow and all DC subsets in the peripher  ...[more]

Similar Datasets

Unknown Gene expression analysis of Cbfb-deficient LSK and GMP
2014-04-05 | GSE55227 | GEO
Unknown The effect of periodontitis - induced trained immunity on the epigenomic profile of mouse bone marrow LSK and GMP cells
2022-04-27 | GSE180025 | GEO
Unknown Tet2-/-Flt3ITD and WT stem and progenitor cells
2015-05-01 | GSE57244 | GEO
Unknown Mx-Cre mediated deletion of SRF in murine hematopoietic LSK (Lin-Sca1+c-Kit+) cells
2010-08-12 | GSE23556 | GEO
Transcriptomics Tet2-/-Flt3ITD and WT stem and progenitor cells
2015-05-01 | E-GEOD-57244 | biostudies-arrayexpress
Unknown RNA-seq profiling of HSC, MPPs and HPC-1s from Phf6-deleted and Phf6-intact mice
2019-02-20 | GSE126316 | GEO
Unknown RNA-sequencing of GFP+ cells 2 days post-transduction of LSK cells with Scr or miR-99 KD lentiviral vectors
2017-07-27 | GSE101902 | GEO
Unknown Mll-AF9 induced changes in gene expression in various hematopoietic cells
2008-02-25 | GSE10627 | GEO
Transcriptomics Mx-Cre mediated deletion of SRF in murine hematopoietic LSK (Lin-Sca1+c-Kit+) cells
2010-08-12 | E-GEOD-23556 | biostudies-arrayexpress
Transcriptomics Transcription profiling of mouse hematopoietic cells (GMP, CMP, CLP and HSC), FACS sorted from wild type and Mll-AF9 knock-ins
2008-04-07 | E-GEOD-10627 | biostudies-arrayexpress