Trasncriptional response of human whole blood following TLR2 or TLR4 ligation in vitro

ABSTRACT: Use of human whole blood for transcriptomic analysis has potential advantages over the use of isolated immune cells for studying the transcriptional response to pathogens and their products. Whole blood stimulation can be carried out in a laboratory without the expertise or equipment to isolate immune cells from blood, with the added advantage of being able to undertake experiments using very small volumes of blood. Toll like receptors (TLRs) are a family of pattern recognition receptors which recognise highly conserved microbial products. Using the TLR2 ligand (Pam3CSK4) and the TLR4 ligand (LPS) human whole blood was stimulated for 0, 1, 3, 6, 12 or 24 hours at which times mRNA was isolated and a comparative microarray was undertaken. A common NFκB transcriptional programme was identi

ORGANISM(S): Homo sapiens

SUBMITTER: Simon Blankley 

PROVIDER: E-GEOD-55375 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress