Kin28 Regulates the Transient Association of Mediator with Core Promoters

ABSTRACT: Mediator is an essential, broadly utilized eukaryotic transcriptional co-activator. How and what it communicates from activators to RNA polymerase II remains an open question. Here we performed genome-wide location profiling of yeast Mediator subunits. Mediator is not found at core promoters but rather occupies the upstream activating sequence (UAS), upstream of the pre-initiation complex. In the absence of Kin28/CDK7 kinase activity, or in cells where the CTD is mutated to replace Ser5 with alanines, however, Mediator accumulates at core promoters together with RNAPII. We propose that Mediator is quickly released from promoters upon Ser5 phosphorylation by Kin28/CDK7, which also allows for RNAPII to escape from the promoter. We took a systematic approach to examine the genome-wide distrib

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Francois Robert 

PROVIDER: E-GEOD-55402 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress