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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse dendritic cells from wild type, CIITA-/- and I-A beta -/- strains representing CIITA-deficient, MHC class II-deficient and control mice

ABSTRACT: Triplicates preparations of RNA from day 10 DC's. Experiment is described in Wong et al 2003 Nat. Immunol.

ORGANISM(S): Mus musculus

SUBMITTER: Athena Wong

PROVIDER: E-GEOD-557 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions.

Wong Athena W AW Brickey W June WJ Taxman Debra J DJ van Deventer Hendrick W HW Reed William W Gao Jian Xin JX Zheng Pan P Liu Yang Y Li Ping P Blum Janice S JS McKinnon Karen P KP Ting Jenny P-Y JP

Nature immunology 20030810 9

The major histocompatibility complex (MHC) class II transactivator (CIITA) is the 'master coactivator' of MHC class II genes. To identify new targets of CIITA, we analyzed cDNA microarrays of dendritic cells (DCs) from CIITA-deficient, MHC class II-deficient and control mice. We found the semaphorin receptor plexin-A1 was expressed in DCs, but not in other immune cells, and was strongly induced by CIITA. RNA interference by short hairpin RNA specific for plexin-A1, but not a single-nucleotide mu ...[more]

PMID: 12910265

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