A genome-wide transcriptome analysis reveals diverse roles of two-component system DraR-K in physiological and morphological differentiation of Streptomyces coelicolor

ABSTRACT: A novel two-component system (TCS) DraR-K was identified previously to play a differential role in antibiotic biosynthesis (ACT, RED and yCPK) of Streptomyces coelicolor M145 on defined minimal medium (MM) conditions. In order to assess whether DraR-K has more global roles, a genome-wide transcriptomic analysis of the wild-type strain M145 and M-bM-^HM-^FdraR-K under the condition of 75 mM glutamine-based MM was performed using DNA microarray. The analysis demonstrated that deletion of draR-K led to the differential expression not only of the biosynthetic gene clusters of ACT, RED and yCPK, but also of other six secondary metabolite biosynthetic clusters, revealing the widely differenital regulation of DraR-K on secondary metabolism of S. coelicolor. Besides, a number of primary metabolism

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Yinhua Lu 

PROVIDER: E-GEOD-55714 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress