Acquisition of the ability to assimilate mannitol by Saccharomyces cerevisiae through dysfunction of Tup1-Cyc8, a general corepressor

ABSTRACT: Saccharomyces cerevisiae normally cannot assimilate mannitol, a promising brown macroalgal carbon source for bioethanol production. To date, the molecular mechanisms underlying this inability remain unknown. Here, we found that cells acquiring mannitol-assimilating ability appeared from wild-type S. cerevisiae strain during prolonged culture in mannitol medium. Our microarray analysis revealed that genes for putative mannitol dehydrogenase and hexose transporters were up-regulated in cells acquiring mannitol-assimilating ability. Take account of our other results including complementation analysis and cell growth data, we demonstrated that this acquisition of mannitol-assimilating ability was due to the spontaneous mutation in the gene encoding Tup1 or Cyc8. Tup1-Cyc8 is the general corepr

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Shigeyuki Kawai 

PROVIDER: E-GEOD-55755 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress